Regulation of Laccase and Cellulase Genes Transcription in Agaricus bisporus

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Production , Purification and Properties of Extracellular Laccase of Agaricus bisporus

Extracellular laccase (EC 1 .14.18.1) of the cultivated mushroom Agaricus bisporus was produced constitutively in defined or complex media. No enzyme induction was found after treatment with cycloheximide or with other potential inducers such as toluidine or xylidine. The enzyme was purified to homogeneity by ammonium sulphate precipitation, ion-exchange chromatography, gel filtration and affin...

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Identification of Two Thermotolerance-Related Genes in Agaricus bisporus

To characterize thermotolerance-related genes in Agaricus bisporus strain 02, we employed differential display PCR (DD-PCR) to analyze total RNA samples extracted from the mycelia grown at different temperatures. Two partial DNA fragments (023-11A and 023-11B) were cloned thus far, the expression of which was correlated with the culturing temperature. The sequences of the two DNA fragments were...

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Endogenous Growth Regulation in Carpophores of Agaricus bisporus.

4. GOODWIN, T. W. 1954. Carotinoids: Their Comparative Biochemistry. Chemical Publishing Co., Inc., N. Y. x plus 356 pp. 5. GOODWIN, T. W. AND S. PHAGPOLNGARM. 1960. Studies in carotenogenesis. 28. The effect of illumination on carotenoid synthesis in French-bean (Phaseolus vulgaris) seedlings. Biochem. J. 76: 197-99. 6. KARRER, P. AND E. JUCKER. 1950. Carotenoids. Elsevier Publ. Co., Inc., N. ...

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Production of ethylene and other volatiles and changes in cellulase and laccase activities during the life cycle of the cultivated mushroom, Agaricus bisporus.

Nine volatile hydrocarbons, as well as methyl chloride, carbonyl sulphide and carbon disulphide, have been identified by mass spectrometry as products of Agaricus bisporus in the compost used in comerical mushroom beds. Of these, only ethylene showed a pattern of production that could be correlated with developmental phases of the crop, high levels being produced whenever fruit bodies were rapi...

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Genome organization and transcription response to harvest of two metallothionein-like genes in Agaricus bisporus fruiting bodies.

Metallothioneins are a class of small cysteine-rich proteins that have been associated with increased tolerance to metal and oxidative stresses in animals, plants, and fungi. We investigated a metallothionein-like (mt-like) gene shown previously to be upregulated in fruiting bodies of the fungus Agaricus bisporus in response to post-harvest storage. Analysis of an A. bisporus genomic DNA cosmid...

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ژورنال

عنوان ژورنال: Journal of the Faculty of Agriculture, Kyushu University

سال: 1998

ISSN: 0023-6152

DOI: 10.5109/24248